Licor removes the need for enzymatic reactions and substrates, with highly stable signals that generate highquality data. Researchers have already downloaded this software over 80000 times. We specialize in creating customized software solutions for virtual school, distance education or a blend of virtual school, distance education, and standard class. Stainfree imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots. Use of proteome profiler arrays with licor detection. Active, participating departments, colleges and schools of howard university that share the department of biologys coreresearch facilities include. Designed for the analysis of nearinfrared western blot images acquired on an odyssey imager, empiria studio is the only western blot analysis software that guides you all the way from validation to statistical analysis for reliable results. It provides access to the machine and analysis software, as well as. The software searches for and discovers the odyssey clx imager on the same lan automatically. Determining the best blocking agent for each target will provide the best chance for successthe electrophoresis buffers maintain consistent ph levels during dna, rna, and other protein separation. Providing hardware, software, professional and managed. Western detection including two color blots and in. Odyssey education partner with industry and world leading technology manufacturers and developers. Nov 09, 2016 pictured here is james, one of our sr.
Maintenance routinemaintenance 15 odysseyfc tableofcontents imager vi. We demod the licor odyssey, biorad chemidoc, and the syngene pxi. Does anyone have experiences with both the licor odyssey scanner system, which uses. Odyssey clx imager page 3 placement in the laboratory the odyssey clx infrared imaging system weighs approximately 33 kg 72 lbs. Simple, free western blot signal quantification software. Page 1 the safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. Instrumentation resource facility school of medicine. At two of the schools, some students may have had access to the odyssey software as. The odyssey fc device and the empiria studio software make reproducible western.
The licor odyssey 9120 delivers accurate and reproducible results with western blot and more. Find the best prices on used licor instruments, equipment, and accessories. If you have questions about how our administrative software solutions would work for your agency, stop by our booth for more information. Imagestudio software learningthesoftware chapter3.
Protein of interest integrated intensity values k counts were normalized to those of gapdh. The licor odyssey fc imaging system is easy to use and the operation is selfexplanatory. We have a passion for innovative technology and great customer service. The instrumentation resource facility irf is an integral component of the research and teaching mission of the university of south carolina school of medicine. Odyssey software will be exhibiting at the open minds tii conference in philadelphia, pa on october 28th and 29th. It is an alternative to standard fluorescence imaging given that it is able to eliminate background fluorescence from samples providing clear and sharp bands. Infrared imaging core school of medicine boston university medical. For more info, see the software specs you can use image studio lite software for basic western blot analysis, but it doesnt operate licor imagers or unicorns. Licor odyssey clx the odyssey clx is the next generation multifunctional imaging platform that can provide a wide range of applications. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel red detection 600 nm are available. View online or download licor odyssey operators manual. Hi everyone, i have western blot pictures, either as a two channel picture or two separate pictures of the two channels 700800nm.
Read this entire manual before using the odyssey fc imager. Used for visualizing images of genes, gene fragments, and gene products. Image studio, image studio lite, and empiria studio software are used with odyssey imagers. Try empiria studio software today and enhance your odyssey workflow with the only western blot analysis software. Odyssey imaging system licor raybiotech preferred scanner scanned images may not be compatible with agilent feature extraction software. Licor odyssey platform for quantitative western blots. Chemidoc imaging systems life science research biorad. Try free image studio lite software for your western blot quantification. Our school recently purchased a western blot documentation system to start. Expert western blot analysis made simple with empiria studio software. The infrared imaging for quantitative analysis core was formed to give investigators access to the licor odyssey clx, an infrared imaging system for quantitative western blot analysis. The odyssey back office system allows you to do a complete stock take or indivdual stock takes according to previously. The odyssey9120 eliminates the guesswork and background fluorescence, allowing for the sharpest digital results needed to make your assays reproducible.
Odyssey clx manuals, application notes, software, and webinars. The odyssey fc imager is intended to be used by qualified personnel. Topbottom before analysis with the odyssey software version 3. The instrument should be placed on a laboratory bench that is sufficiently sturdy to bear its weight. Image studio software work area folder best practices canning a mouse on. Foxo transcription factors support oxidative stress. When installing the odyssey software on a computer that has the older odyssey software installed, the installation application needs to be run once to remove the older software and then again to install the latest odyssey software. This protocol is designed for the odyssey infrared imaging system by licor. Which is the best and more sensitive western blot imaging. Using software that isnt supported by licor like photoshop, paint, microsoft photo editor, imagej, and others can modify your raw image file and affect your data. Facilities and equipment howard university department of. View online or download odyssey licor operators manual.
Chemidoc imagers offer bestinclass performance with ease of use for visible light rgb and far rednear infrared frnir fluorescence and chemiluminescence detection and all general gel documentation applications. With odyssey clx, you can get consistent, reproducible digital images, without the hassles and unpredictability of film. You can use multiple colors at the same time to detect mouse and rabbit antibodies. If you experience connection problems, wait several minutes and then click connect again. Laser safety the center for devices and radiological health cdrh was established in october, 1982, by the u. Licor image studio software for accurate data quantification. The licor odyssey infrared imager is an awesome instrument for western blot applications. It gives a basic overview of the functionality and i run through the analysis of a gel. Odyssey applications featuring irdye infrared dyes infrared dyes are a key performance advantage of the odyssey system. Image studio software can be used for quick acquisition and organization of images from most licor imaging systems, including the odyssey clx. The next time you connect, the software will automatically detect the odyssey clx imager. Available as a free download or supplied on disc with your order at a small cost. The licor odyssey allows us to veiw our western blots quickly and efficiently.
Empiria studio uses workflows to minimize usertouser variation, compute statistical values, and provides more extensive analysis options. Free western blot quantification software licor biosciences. Each of the six pillars of the services and solutions of this 360 degree continuum, both separately and collectively, addresses the challenges posed by the everchanging cyberthreat landscape in all key. Incell western protocol incell western assays icw also known as incell elisa ice allow researchers a simple and rapid assay method for detection of biomarkers and signaling proteins in whole cells using antibodies. The odyssey tm 360 o our approach to information security which ensures the effective management of organizational cyber risk. The text above is not a piece of advice to uninstall image studio lite 5. The consistent digital images produced by the imaging system cut out the hassle of the film while providing clear nearinfrared results. Used other licor odyssey imager for sale dotmed listing. Can be used to distinguish between normal and cancer cells. Our school recently purchased a western blot documentation system to start slowly moving away from film. Download image studio lite software licor biosciences.
Image studio lite software is compatible with mac and windows. The odyssey clx is the next generation multifunctional imaging platform that can provide a wide range of applications. The intensity of the bands was quantified using licor odyssey software and represented by bar graphs. This text only contains detailed instructions on how to uninstall image studio lite 5. The company produced games for the amiga and the nintendo entertainment system nes. Cell lysates were extracted 48 h posttransfection and immunoblotted with respective antibodies. We bought the odyssey fc imaging system from licor a year ago and we are more than happy with it. Qwb data was analysed using odyssey software as per manufactures guidelines and as previously described. It provides access to the machine and analysis software, as well as training for new users, and. Which is the best and more sensitive western blot imaging system. Blots were washed in pbs and then water before image acquisition on the licor odyssey. It has two lasers, which operate at 680 and 780nm, for the detection of infrared fluorescent dyes. The odyssey 9120 eliminates the guesswork and background fluorescence, allowing for the sharpest digital results needed to make your assays reproducible. The experiments were repeated three times n 3, and representative images are presented.
Its technology allowed companies to manage multiple mobile operating systems at a detailed level, including functions such as inventory collection. Here is a detailed instruction, you may follow that step by step. Odyssey software was a computer game developer founded in 1987 in eastham, massachusetts by art v. Computer software cables cords manual chemiluminescence and. In the prompt box that opens, select browse from the right side. Odyssey software delivers robust capabilities for the management and security of todays constantly evolving mobile enterprise.
This is necessary because the raw images consist of 16 bit tiff images. Image optimization most blots after scanning will require some adjustments in order to visualize the relevant portions of the images on the computer monitor. Troubleshooting for the odyssey infrared imaging system. Odyssey clx infrared imaging system operators manual. Licor families enjoyed a saturday at roca berry farm on september 24, despite the occasional shower and few brief downpours. Does anybody have a lot of experience with image studio lite by li. College of arts and sciences biology, chemistry, psychology, physics, and mathematics, the college of engineering, architecture, and computer sciences department of chemical engineering and. Licor image studio lite free densitometry software.
Image studio is used for pearl imagers and cdigit blot scanner. Quantification of protein band density in xray films from ecl western blots. Quantification of protein band density in xray films from. This product model number 2800 is a cemarked product. This is a quick look at the free densitometry software from licor. The university of texas health science center at houston and childrens memorial hermann hospital provide facilities and other resources to the principal investigator and his research team to undertake and complete the proposed research project successfully. The simple interface puts highquality data at your fingertips with a few simple clicks and without manual camera adjustments or multiple exposures. Licor odyssey gel imaging scanner university of washington. This video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. I really want a licore odyssey clx but cant fit it in the budget. Get consistent, reproducible digital images, without the hassles and unpredictability of film. The macroscopic imaging center facility also contain an imagequant las 500 detection system for chemiluminescence, fluorescence and colorometric measurements from microtiter format plates and a licor odyssey clx near ir scanner for dual color western blot analysis. Image acquisition and file organization software for use with licor imaging systems.
Using the licor odyssey scanner to image the qplex array. Odysseys definition of odysseys by the free dictionary. Being able to double label with the 700 and 800 ir laser also allows us to save blots and time. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Data was graphed and statistical comparisons carried out using graphpad prizm as previously described. Welcome to the department of microbiology infrared imaging for quantitative analysis core. Protein bands were visualized following incubation with licor fluorescent secondary antibodies licor, catalog no. The only western blot analysis software that guides you from validation through statistical analysis for reliable results. Empiria studio software is designed for the analysis of nearinfrared western blot images acquired on an odyssey imager.
Etf sponsored research program facilities and resources. Odyssey clx application protocols manual vanderbilt university. The application button provides options for importing and exporting images, reporti. Accurate, replicable western blots with the odyssey clx. Licor biosciences, odyssey infrared imaging system b446. Image studio software features are designed to preserve the integrity of your raw quantitative image data, even if you adjust the display. Since the odyssey is a two channel instrument it can record data for either wavelength separately or both wavelengths simultaneously. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Does anyone have experiences with both the licor odyssey.
Fluorescent wb protocol using iblot fast transfer and licor odyssey. Manuals, software information, and webinars are also listed. Licor odyssey clx college of education and human sciences. Although the lite version is free, there is a more comprehensive paid version of the software that. Odyssey infrared imaging system operators manual version 3. A guide to modern quantitative fluorescent western blotting with troubleshooting strategies.
Biosurplus has the most comprehensive and available list of used laboratory equipment online. When initial screen has loaded, click on file and select new from the dropdown menu. The consistent digital images produced by the imaging system cut out the hassle of film, while providing clear near infrared results. Backed by decades of mobile device management expertise and an. Odyssey software provided mobile device management and software development tools to enterprise companies either directly primarily through its athena product or through partner solutions. Additionally, i have used this for imaging my crystal violet stained plates. A guide to modern quantitative fluorescent western. Licor biosciences pioneering family of irdye infrared dyes are synthesized with reactive functional groups that enable easy covalent coupling to antibodies and other biomolecules.
Fortunately, a new party barn was open for our event that allowed for some additional together time during lunch while the weather front moved through roca. View and download odyssey licor operators manual online. Total protein analysis as a reliable loading control for. The odyssey fellowship program supports the training and research efforts of dedicated scientists at the beginning of their career, while the outstanding research publication is an award given yearly to recognize the achievements of postdoctoral fellows for work accomplished at the md anderson cancer center. After providing your information, youll get an email with a link to download image studio lite software. Licor, incell western, mpx, newblot, odyssey, and irdye are registered. When quantitative western blot detection or nearinfrared fluorescent imaging is needed, the bioagilytix team knows the licor platform will deliver the sensitivity and linearity required to yield accurate results.
This video tutorial demonstrates the image studio 4. Odyssey software mobile device management wikipedia. Odyssey clx application and technical notes show the versatility of the clx. Electronic technicians, working on an odyssey clx, our nearinfrared molecular imaging platform. There are certainly many different ways to both organize and retrieve. Licor lab instruments, equipment, and accessories biosurplus. The odyssey software is essential to operate the odyssey classic loggers and manage the resulting data. For conformity information, contact licor support at biosupport. The xtreem logger xtract android app available for free download. Image studio software is compatible with most licor imaging systems, including the odyssey imaging systems, pearl imaging system, and cdigit blot.
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